- The expression of ionotropic receptor in drosophila larvae cool
cells alters temperature sensitivity in hypertonic environment
- Hua Bai
- studying cool cells in larvae
- larva prefer to stay at 24C
- there are cool and warm cells in the DOG (?) that help the larva theromotax
- A and B type cool cells
- IR25a and IR93a are the cool receptors
- IR21a is the tuning center for cooling cells
- hyptertonic conditions - put larva in sucrose causes stress and loss of B cool cells
- this is an issue because this is how most people collect larva for experiments
- overexpression of IR93a strengthens expression of B cells
- Essential elements of radical pair magnetosensitivity in Drosophila
- Adam Bradlaugh
- radical pair mechanism of magnetism…
- expressing cryptochrome (CRY) and magnetic field causes cell to fire more
- if you load the cell with FAD, cell is more potentiated and demonstrated that FAD is helping to make the radical pair in cells
- with enough FAD, the cell can become sensitive to magnetic fields
- FAD binds to CRY and this might be the evolutionary mechanism for magneto sensing
- experiments were done in larva and they would change magnetic fields and larva would change orientation in response.
- Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion
- Amanda J. Gonzalez-Segarra (Kristin Scott lab)
- regulate thirst and hunger through ISNs in the SEZ
- thirst = water
- hunger = sugar
- 104 post synaptic targets all in the SEZ and SMP
- pulled out one called the Bilateral T shaped cell
- ISNs inhibit the bilateral T shaped cell
- they used ArcLight — not sure what this tool is
- sugar and water ingestion is regulated by BiT and CCAP cells
- Biophysical and molecular bases of activation and adaptation in Johnston’s organ neurons
- Stephen Holtz (Rachel Wilson Lab)
- 500 JONs that express 2 different TRP channels
- recording JONs with ephys while manually moving the antenna with a piezo
- JONs input are the stretching of the cilia in the antenna
- neighboring JONs have similar inputs but different adaptation rates
- there is input driven by tension of the cilia and this results in transduction
- tension causes spiking in the JONs then the tension has to loosen again
- but just like a rubber band that stretches and then returns to baseline there is an overshoot in the loosening and then they have to stretch a little more again which causes another little bit of spiking in the JONs
- nan-inv and nompC required for diversity of responses in JONs and the retension behavior
- Sensory correlates of electrotaxis in the Drosophila larva
- David Tadres
- asking if larva can electrotax and move to negative side of the chamber
- basically used a glorified electrophoresis box and they could change the polarity of the box
- when you flip the polarity of the box, the larva switches direction
- Gr66a required for the electrotaxis
- can detect changes in the direction of the electric field
- depolarizes when facing the anode - like a “stop” neuron