transmission electron microscopy is slow. each slice gets placed on a long piece of tape. slice goes over hole in tape which acts as an aperture. each slice has a unique barcode with it. This is an automated process that speeds up dataset collection dramatically
references
Phelps, J. S., Hildebrand, D. G. C., Graham, B. J., Kuan, A. T., Thomas, L. A., Nguyen, T. M., Buhmann, J., Azevedo, A. W., Sustar, A., Agrawal, S., Liu, M., Shanny, B. L., Funke, J., Tuthill, J. C., & Lee, W.-C. A. (2021). Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy. Cell, 184(3), 759-774.e18. https://doi.org/10.1016/j.cell.2020.12.013