when presenting a range of stimuli to the antenna of the fly, Patella.Wilson2018 recorded JON response types and performed unsupervised clustering of single pixel values of their calcium imaging datasets. Groups of correlated pixels were clustered and revealed a map of responses spatially, where low frequencies activated medial neurons spanning to high frequency responses laterally. Each response type has a range of amplitudes they were responsive too and there are various adaptation profiles for each type. The center frequencies of each type range from 0-600hz (each acting as a somewhat bandpass filter)
notably, courtship song recruits all JON types.
similar tonotopy was found in the AMMC and WED. However, the AMMC showed over representation of low frequencies and the wedge over represented extreme frequencies. The wedge also had much narrower tuning of each individual response. This also led to a more orderly tonotopic representation in the wedge.